5. Alkhamaiseh S. I., Taher M., Farediah A., Qaralleh H., Althunibat O.Y., Deny Susanti D. and Solachuddin Jauhari Arief Ichwan S. J. (2012). The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark). Pakistan Journal of Pharmaceutical Sciences, 25 (3): 555-563.
Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes
- Research Summary
Medicinal plants are being used extensively in Jordanian traditional medicinal system for the treatment of diabetes symptoms. Twenty one plant samples were collected from different Jordanian locations and used for antioxidant evaluation. The level of antioxidant activity was determined by DPPH and ABTS assays in relation to the total phenolic contents of the medically used parts. The most frequently used plant parts as medicines were fruit, shoot and leaves. The total phenolic contents of methanol and aqueous extracts, from plants parts, ranged from 6.6 to 103.0 and 3.0 to 98.6 GAE mg g(-1) of plant part dry weight, respectively. DPPH-TEAC of the methanol extracts of plants parts were varied from 4.1 to 365.0 mg g(-1) of plant dry weight versus 0.6 to 267.0 mg g(-1) in aqueous extracts. Moreover, the mean values of ABTS*- (IC50) varied from 6.9 to 400.0 microg dry weight mL(-1) ABTS in methanol extracts versus 9.8 to 580.5 microg mL(-1) in aqueous extracts. According to their antioxidant capacity, the plants were divided into three categories: high (DPPH-TEAC > or = 80 mg g(-1) ), (i.e., Punica granatum peel, Quercus calliprinos leave, Quercus calliprinos fruit, Cinchona ledgeriana and Juniperus communis leave), moderate (DPPH-TEAC range 20-80 mg g(-1)) (i.e., Salvia fruticosa shoot, Crataegus azarolus stem, Crataegus azarolus leave, Varthemia iphionoides shoot, Artemisia herba-alba shoot, Thymus capitatus shoot, Morus nigra leaves and Arum palaestinum leaves) and low antioxidant plants (DPPH-TEAC < 20 mg g(-1)), (i.e., Matricaria aurea shoot, Artemisia judaica shoot, Teucrium polium shoot, Pinus halepenss pollen grains, Sarcopoterium spinosum root, Crataegus azarolus fruit, Inula viscose shoot and Achillea fragrantissima shoot). The antioxidant activity of these plant's extracts and their potential rule in radical scavenging agreed with their potential use by Jordanian population as a traditional anti-diabetic agents.
- Research link
- key words
antioxidant, DPPH, ABTS, TEAC, Polyphenols, GAE, Jordan
In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
- Research Summary
The antioxidant and antiproliferative activities of aqueous and organic extracts from three
sea cucumber species, Holothuria scabra, Holothuria leucospilota and Stichopus
chloronotus were studied. The result showed that aqueous extract of H. leucospilota
contains the highest level of total phenols (9.7 mg GAE/g), while the lowest level was
obtained from organic extract of H. scabra (1.53 mg GAE/g). An aqueous extract of S.
chloronotus was the most efficient extract in scavenging of DPPH free radical, giving IC50=
2.13 mg/ml. The aqueous extracts (50mg/ml) of H. scabra, H. leucospilota and S.
chloronotus exhibited high antioxidant activities (77.46%, 64.03% and 80.58%, respectively)
against linoleic acid free radical. Only an aqueous extract of S. chloronotus inhibited growth
of C33A (human cervical cancer) and A549 (human non-small lung carcinoma) cancer
- Research link
- key words
Sea cucumber, Antioxidant, Antiproliferation.
Antibacterial activity in vitro of Thymus capitatus from Jordan.
- Research Summary
This study was carried out to evaluate the antibacterial activity of aqueous and organic
extracts of Thymus capitatus L.(Lamiaceae) leaves and stems. Dried ground powder leaves
and stems were extracted with water (aqueous extracts), ethanol, dichloromethane and
hexane (Soxhlet extracts). The antibacterial activity of these extracts was evaluated against
bacteria using disc diffusion method. The result obtained showed that the leaves had
stronger antibacterial activity than the stems extracts. The ethanolic extract had the highest
yield products and the high antibacterial activity than all other solvents. The results suggest
that essential oil as non-polar organic compounds could be the main active compounds in
this plant. Therefore the antibacterial activity of leaves ethanol extracts (LEE) was compared
with essential oils leaves extracts (LEO) of T. capitatus. The LEO showed greater …
- Research link
- key words
Thymus capitatus, antibacterial, soxhlet extraction, essential oil.
1. Almustafah, A. and Althunibat, O.Y., (2009). Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pakistan Journal of Boilogical Sciences,11 (3): 351-358.
2. Althunibat O.Y., Ridzwan B.H., Taher M., Daud J.M., Ikeda M.A., and Zali B.I. (2009). In Vitro Antioxidant and Antiproliferative Activities of Three Malaysian Sea Cucumber Species. European Journal of Scientific Research, 37 (3): 376-387.
3. Qaralleh H.N., Abboud M.M., Khleifat K., Tarawneh K.A., and Althunibat O.Y., (2009). Antibacterial activity in vitro of Thymus capitatus from Jordan. Pakistan Journal of Pharmaceutical Sciences, 2 (3): 247-251
Althunibat, O. Y., Almustafah, A., Tarawneh K., Khlefat K., Ridzwan B. H., and Qaralleh H. (2010). Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats. Process Biochemistry, 45: 581-585.
5. Alkhamaiseh S. I., Taher M., Farediah A., Qaralleh H., Althunibat O.Y., Deny Susanti D. and Solachuddin Jauhari Arief Ichwan S. J. (2012). The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark). Pakistan Journal of Pharmaceutical Sciences, 25 (3): 555-563.
Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes
- Research Summary
Medicinal plants are being used extensively in Jordanian traditional medicinal system for the treatment of diabetes symptoms. Twenty one plant samples were collected from different Jordanian locations and used for antioxidant evaluation. The level of antioxidant activity was determined by DPPH and ABTS assays in relation to the total phenolic contents of the medically used parts. The most frequently used plant parts as medicines were fruit, shoot and leaves. The total phenolic contents of methanol and aqueous extracts, from plants parts, ranged from 6.6 to 103.0 and 3.0 to 98.6 GAE mg g(-1) of plant part dry weight, respectively. DPPH-TEAC of the methanol extracts of plants parts were varied from 4.1 to 365.0 mg g(-1) of plant dry weight versus 0.6 to 267.0 mg g(-1) in aqueous extracts. Moreover, the mean values of ABTS*- (IC50) varied from 6.9 to 400.0 microg dry weight mL(-1) ABTS in methanol extracts versus 9.8 to 580.5 microg mL(-1) in aqueous extracts. According to their antioxidant capacity, the plants were divided into three categories: high (DPPH-TEAC > or = 80 mg g(-1) ), (i.e., Punica granatum peel, Quercus calliprinos leave, Quercus calliprinos fruit, Cinchona ledgeriana and Juniperus communis leave), moderate (DPPH-TEAC range 20-80 mg g(-1)) (i.e., Salvia fruticosa shoot, Crataegus azarolus stem, Crataegus azarolus leave, Varthemia iphionoides shoot, Artemisia herba-alba shoot, Thymus capitatus shoot, Morus nigra leaves and Arum palaestinum leaves) and low antioxidant plants (DPPH-TEAC < 20 mg g(-1)), (i.e., Matricaria aurea shoot, Artemisia judaica shoot, Teucrium polium shoot, Pinus halepenss pollen grains, Sarcopoterium spinosum root, Crataegus azarolus fruit, Inula viscose shoot and Achillea fragrantissima shoot). The antioxidant activity of these plant's extracts and their potential rule in radical scavenging agreed with their potential use by Jordanian population as a traditional anti-diabetic agents.
- Research link
- key words
antioxidant, DPPH, ABTS, TEAC, Polyphenols, GAE, Jordan
In vitro antioxidant and antiproliferative activities of three Malaysian sea cucumber species
- Research Summary
The antioxidant and antiproliferative activities of aqueous and organic extracts from three
sea cucumber species, Holothuria scabra, Holothuria leucospilota and Stichopus
chloronotus were studied. The result showed that aqueous extract of H. leucospilota
contains the highest level of total phenols (9.7 mg GAE/g), while the lowest level was
obtained from organic extract of H. scabra (1.53 mg GAE/g). An aqueous extract of S.
chloronotus was the most efficient extract in scavenging of DPPH free radical, giving IC50=
2.13 mg/ml. The aqueous extracts (50mg/ml) of H. scabra, H. leucospilota and S.
chloronotus exhibited high antioxidant activities (77.46%, 64.03% and 80.58%, respectively)
against linoleic acid free radical. Only an aqueous extract of S. chloronotus inhibited growth
of C33A (human cervical cancer) and A549 (human non-small lung carcinoma) cancer
- Research link
- key words
Sea cucumber, Antioxidant, Antiproliferation.
Antibacterial activity in vitro of Thymus capitatus from Jordan.
- Research Summary
This study was carried out to evaluate the antibacterial activity of aqueous and organic
extracts of Thymus capitatus L.(Lamiaceae) leaves and stems. Dried ground powder leaves
and stems were extracted with water (aqueous extracts), ethanol, dichloromethane and
hexane (Soxhlet extracts). The antibacterial activity of these extracts was evaluated against
bacteria using disc diffusion method. The result obtained showed that the leaves had
stronger antibacterial activity than the stems extracts. The ethanolic extract had the highest
yield products and the high antibacterial activity than all other solvents. The results suggest
that essential oil as non-polar organic compounds could be the main active compounds in
this plant. Therefore the antibacterial activity of leaves ethanol extracts (LEE) was compared
with essential oils leaves extracts (LEO) of T. capitatus. The LEO showed greater …
- Research link
- key words
Thymus capitatus, antibacterial, soxhlet extraction, essential oil.
1. Almustafah, A. and Althunibat, O.Y., (2009). Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pakistan Journal of Boilogical Sciences,11 (3): 351-358.
2. Althunibat O.Y., Ridzwan B.H., Taher M., Daud J.M., Ikeda M.A., and Zali B.I. (2009). In Vitro Antioxidant and Antiproliferative Activities of Three Malaysian Sea Cucumber Species. European Journal of Scientific Research, 37 (3): 376-387.
3. Qaralleh H.N., Abboud M.M., Khleifat K., Tarawneh K.A., and Althunibat O.Y., (2009). Antibacterial activity in vitro of Thymus capitatus from Jordan. Pakistan Journal of Pharmaceutical Sciences, 2 (3): 247-251
Althunibat, O. Y., Almustafah, A., Tarawneh K., Khlefat K., Ridzwan B. H., and Qaralleh H. (2010). Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats. Process Biochemistry, 45: 581-585.